BCIP/NBT Membrane Alkaline Phosphatase Substrate from Rockland Immunochemicals, Inc.

Description

BCIP/NBT Membrane Alkaline Phosphatase Substrate is an artificially manufactured chromogenic substrate for use in sensitive colorimetric assays. Alkaline phosphatase hydrolyzes the BCIP to 5-bromo-4-chloro-3-indole, which is oxidized by oxygen in the atmosphere, yielding a blue dye. In the presence of NBT, an insoluble dark blue precipitate is formed after a reduction reaction instead

Product Specs
ItemBCIP/NBT Membrane Alkaline Phosphatase Substrate
CompanyRockland Immunochemicals, Inc.
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberNBT-100
Quantity100 mL
Conjugate/Tag/LabelUnconjugated
ApplicationsWestern Blot
PuritypH: 9.28 +/- 0.1 QC Raw Material: PASS Absorbance check of final product: PASS Performance Data per Immunoblot: PASS
FormatLiquid - clear to pale yellow
Concentration1X
StorageStore container at 4° C prior to opening. Protect BCIP/NBT Membrane Alkaline Phosphatase Substrate from moisture and light. No special shipping conditions or precautions are required

Specifications/Features

Application Note: BCIP/NBT Alkaline Phosphatase Membrane Substrate is a useful detection reagent for immunoblotting. The substrate is ready to use. To use: Wash the membrane after incubation with the alkaline phosphatase conjugate secondary reagent. Apply sufficient substrate to cover the membrane. Color (purple-turquoise, varies with pH) will develop within 30 minutes. Wash, dry membrane and image.

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Rockland Immunochemicals, Inc.

P.O. Box 5199

Limerick, Pennsylvania 19468

United States

Phone: (484) 791-3823
Fax: (484) 369-8654

Company Profile

Website: https://www.rockland.com

(1) Breakthrough infections by SARS-CoV-2 variants boost cross-reactive hybrid immune responses in mRNA-vaccinated Golden Syrian HamstersbioRxivJune 28, 2023Juan García-Bernalt García-Bernalt Diego, Gagandeep Singh, Sonia Jangra, Kim Handrejk, Manon Laporte, Lauren A. Chang, Sara S. El Zahed, Lars Pache, Max W. Chang, Prajakta Warang, et al.proteins","Chemicals, peptides and recombinant proteins" BCIP/NBT Membrane Alkaline phosphatase substrate,Rockland,NBT-100 BNT162b2,Recovered from the Mt Sinai Hospital vaccination site,N/A Gibco Advanced DMEM/F12,Thermo Fisher

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BCIP/NBT Membrane Alkaline Phosphatase Substrate from Rockland Immunochemicals, Inc.

Description

Specifications/Features

Rockland Immunochemicals, Inc.

Citations (1)

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BCIP/NBT Membrane Alkaline Phosphatase Substrate from Rockland Immunochemicals, Inc.

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